Induction of both thymidine and deoxycytidine kinase activity by herpes viruses.

Herpesvirus proteins: induction of nucleoside phosphotransferase activity after herpes simplex virus infection.

After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activity. Phosphotransferase activity is not induced in cells lacking both thymidin...

PCR detection of thymidine kinase gen of latent herpes simplex Virus type 1 in mice trigeminal ganglia

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

Isolation of cis-acting vaccinia virus DNA fragments promoting the expression of herpes simplex virus thymidine kinase by recombinant viruses.

Recombinant TK- vaccinia viruses containing the pBR322 sequence inserted in either orientation within the coding sequence of the viral thymidine kinase gene were constructed. They were characterized by genomic analysis, hybridization studies, reversion to wild-type virus by in vivo recombination, and rescue from their genomes of plasmids which contained all or parts of the pBR322 sequence. TK- ...

Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1

Deoxycytidylate Deaminase Activity of Simian Virus 40-infected Cell Cultures1

ney cell extracts with dCTP also exhibit as much activity as do those from vacciniaand Simian Virus 40 (SV40)-infected cultures, respectively. Extracts prepared with dCTP from vacciniaor herpes simplexinfected mouse embryo and mouse kidney cultures have no more than 1.7 times the deoxycytidylate deaminase activity of uninfected cell extracts; however, thymidine kinase activity of virus-infected...